Test Code TRICH Trichomonas PCR
Additional Codes
Compendium Code(Iatric): L700.7010
Performing Laboratory
St. Lukes Hospital Laboratory
Methodology
APTIMA Combo 2
Specimen Requirements
Specimen must arrive within 4 days of collection.
Submit only 1 of the following specimens:
ThinPrep Pap Vial
1. Physician may recover specimen from different areas, such as posterior fornix, ectocervix, and endocervix using cervical broom.
2. Do not use lubricant on speculum.
3. Visualize cervix with a speculum.
4. Obtain an adequate sampling from endocervix using an
endocervical broom device. Insert broom into cervix until only
bottom-most fibers are exposed. Slowly rotate one quarter or one
half turn in 1 direction. Do not over-rotate. Additional rotation
may cause bleeding and contaminate specimen.
5. Rinse broom in ThinPrep® Pap Test PreservCyt® Solution vial by rotating device in solution 10 times while pushing against PreservCyt® vial wall. Swirl broom VIGOROUSLY to further release material. Discard broom.
6. Tighten cap so torque line on cap passes torque line on vial.
7. Label vial with patient’s name (first and last), date of birth, date and actual time of collection, and type of specimen.
8. Forward promptly at ambient temperature.
1. Note: Specimen source is required on request form for processing.
Vaginal Swab
1. Use the APTIMA vaginal collection swab (Orange).
2. Carefully insert the swab into the vagina about 2 inches
past the introitus and gently rotate the swab for 10-20 seconds
making sure the swab touches the walls of the vagina so moisture is
absorbed by the swan.
3. Withdraw swab without touching the skin.
4. Remove cap from transport media and place swab into transport
tube. Break swab at score line and replace cap.
5. Label tube with patient’s name (first and last), date and
actual time of collection, and type of specimen.
6. Send specimen at room temperature. Maintain
sterility and forward promptly.
Note: Specimen source is
required on request form for processing.
Urine (Male or female)
1. Patient should not have urinated within 1 hour prior to specimen
collection.
2. Collect first 20 mL to 30 mL of
voided urine in a plastic, sterile, preservative-free
container. (Midstream collection is not
acceptable.)
3. Transfer 2 ml of urine into Yellow APTIMA transport tube using the disposable pipette. The fluid level should be between the black fill lines on the tube.
4. Label container with patient’s name (first and last), date and actual time of collection, and type of specimen.
5. Send specimen at room temperature or refrigerated.
Maintain sterility and forward promptly.
Note: Specimen source is
required on request form for processing.
Reference Values
Negative
Limitations:
1. A negative test result does not exclude the possibility
of infection because test results may be affected by improper
specimen collection, technical error, specimen mix-up, concurrent
antibiotic therapy, or the small number of organisms in the
specimen may be below the sensitivity of the test.
2. Performance has not been evaluated in pregnant women or women
under the age of 14.
3. This system should not be used to assess therapeutic
success or failure since nucleic acids from Trichomonas
vaginalis may persist following antibmicrobial
therapy.
Day(s) Test Set Up
Monday through Friday
Test Classification and CPT Coding
CPT: 87661