Test Code HPVHR Human Papillomavirus (HPV) Detection-High Risk Types
Additional Codes
Compendium Code (Iatric): L690.0013
Performing Laboratory
St. Lukes Hospital Laboratory
Methodology
APTIMA HPV Amplified Probe
Specimen Requirements
Container/Tube: ThinPrep® vial containing at least 8 mL of PreservCyt® solution
Specimen Volume: Cervical swab
Collection Instructions:
1. Physician may recover specimen from different areas, such as posterior fornix, ectocervix, and endocervix using cervical broom.
2. Do not use lubricant on speculum.
3. Visualize cervix with a speculum.
4. Obtain an adequate sampling from endocervix using an
endocervical broom device. Insert broom into cervix until only
bottom-most fibers are exposed. Slowly rotate one quarter or one
half turn in 1 direction. Do not over-rotate. Additional rotation
may cause bleeding and contaminate specimen.
5. Rinse broom in ThinPrep® Pap Test PreservCyt® Solution vial by rotating device in solution 10 times while pushing against PreservCyt® vial wall. Swirl broom VIGOROUSLY to further release material. Discard broom.
6. Tighten cap so torque line on cap passes torque line on vial.
7. Label vial with patient’s name (first and last), date of birth, date and actual time of collection, and type of specimen.
8. Forward promptly at ambient temperature.
Note: 1. Specimen source is required.
2. Bag ThinPrep® specimens individually as they have a tendency to leak during transport. Place labels on vial and bag.
Reference Values
Negative for types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68
Positive results will be reflexed for HPV 16/18/45 typing if requested.
This assay has not been approved or validated for any site other than uterine cervix specimens. This assay has not been evaluated for the management of women with prior cytological or histological abnormalities, women who are pregnant, post menopausal, HIV-positive, or have a history of sexually transmitted infection. Performance of this assay has not been adequately established for HPV-vaccinated individuals. Correlation of results with clinical findings is recommended.
Day(s) Test Set Up
Weekedays
Test Classification and CPT Coding
CPT: 87621
LOINC: 59420-0
Reporting Title
High Risk HPV DNA Detection
Specimen Transport Temperature
Ambient 10 weeks/Refrigerate NO/Frozen NO