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Test Code HPVHR Human Papillomavirus (HPV) Detection-High Risk Types

Additional Codes

Compendium Code (Iatric): L690.0013

Performing Laboratory

St. Lukes Hospital Laboratory

Methodology

APTIMA HPV Amplified Probe

Specimen Requirements

Container/Tube: ThinPrep® vial containing at least 8 mL of PreservCyt® solution

Specimen Volume: Cervical swab

Collection Instructions:

1. Physician may recover specimen from different areas, such as posterior fornix, ectocervix, and endocervix using cervical broom.

2. Do not use lubricant on speculum.
3. Visualize cervix with a speculum.
4. Obtain an adequate sampling from endocervix using an endocervical broom device. Insert broom into cervix until only bottom-most fibers are exposed. Slowly rotate one quarter or one half turn in 1 direction. Do not over-rotate. Additional rotation may cause bleeding and contaminate specimen.

5. Rinse broom in ThinPrep® Pap Test PreservCyt® Solution vial by rotating device in solution 10 times while pushing against PreservCyt® vial wall. Swirl broom VIGOROUSLY to further release material. Discard broom.

6. Tighten cap so torque line on cap passes torque line on vial.

7. Label vial with patient’s name (first and last), date of birth, date and actual time of collection, and type of specimen.

8. Forward promptly at ambient temperature.

Note: 1. Specimen source is required.

2. Bag ThinPrep® specimens individually as they have a tendency to leak during transport. Place labels on vial and bag.

Reference Values

Negative for types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68

Positive results will be reflexed for HPV 16/18/45 typing if requested.

 

This assay has not been approved or validated for any site other than uterine cervix specimens. This assay has not been evaluated for the management of women with prior cytological or histological abnormalities, women who are pregnant, post menopausal, HIV-positive, or have a history of sexually transmitted infection. Performance of this assay has not been adequately established for HPV-vaccinated individuals. Correlation of results with clinical findings is recommended.

Day(s) Test Set Up

Weekedays

Test Classification and CPT Coding

CPT: 87621

LOINC: 59420-0

Reporting Title

High Risk HPV DNA Detection

Specimen Transport Temperature

Ambient 10 weeks/Refrigerate NO/Frozen NO