Test Code GYN Cytology, Gynecologic, Pap Smear (ThinPrep®)
Performing Laboratory
St. Lukes Hospital Laboratory
Methodology
ThinPrep® Pap Test
Specimen Requirements
1. Physician may recover specimen from different areas, such as posterior fornix, ectocervix, and endocervix using cervical broom.
2. Do not use lubricant on speculum.
3. Visualize cervix with a speculum.
4. Obtain an adequate sampling from endocervix using an endocervical broom device. Insert broom into cervix until only bottom-most fibers are exposed. Slowly rotate one quarter or one half turn in 1 direction. Do not over-rotate. Additional rotation may cause bleeding and contaminate specimen.
5. Rinse broom in ThinPrep® Pap Test PreservCyt® Solution vial by rotating device in solution 10 times while pushing against PreservCyt® vial wall. Swirl broom VIGOROUSLY to further release material. Discard broom.
6. Tighten cap so torque line on cap passes torque line on vial.
7. Label vial with patient’s name (first and last), date of birth, date and actual time of collection, and type of specimen.
8. Forward promptly at ambient temperature.
Note: Complete a “Request for Specimen Analysis” in “Request Forms” in “Special Instructions” including patient’s name (first and last), date of birth, date and actual time of collection, requesting physician name, collector’s name, pertinent clinical history including (last menstrual period, gynecologic surgery, previous cytology results, etc.), and information. Forward it with the specimen.
Reference Values
See interpretive data reported using current Bethesda 2001 format.
Human Papillomavirus (HPV) high-risk and HPV high-risk/low-risk testing is available reflexively on atypical squamous cells of undetermined significance (ASCUS) Pap smears, and others, as requested.
Interfering Substances:
Only lubricants approved for ThinPrep® testing are advisable. Call St. Luke’s Hospital Cytology Laboratory at 218-249-5315 for listings.
Day(s) Test Set Up
Monday through Friday
Test Classification and CPT Coding
G0123